Quick-freeze deep-etch images show a more three dimensional view of acidosomes surrounding a phagosome. Acidosomes are unique in that they contain indentations of their membrane. The lumen has a honeycombed appearance, also unique to these vesicles. Their E-face is almost free of IMPs while their P-face has many large particles which leave pits in the E-face. Acidosomes maintain a close association with the phagosome membrane. In this micrograph the difference between the lumens of pretrichocyst vesicles and acidosomes can be compared. TEM taken on 5/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X. Bar = 0.5?m. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Process: Vesicle fusion, Phagosome acidification
Author: Richard Allen (University of Hawaii)
Source: The Cell: An Image Library
