Stable cell lines carrying a reporter gene (a tag or a fluorescent protein) cassette using CRISPR targeted genome editing via the HDR pathway is a powerful tool for monitoring endogenous target gene expression or biological processes without disrupting the target gene in vitro.
Based on years of experience and in-depth investigation, scientists at Creative Biogene can target specific DNA sequences and induce a double-stranded break, taking advantage of recombination to create a synthetic genetic genome of a host. An expression cassette containing reporter and resistance genes was knocked into the safe-harbor site or any other sites in host cells. Gene insertion at a safe-harbor site allows stable gene expression without any adverse effects on the fitness of the engineered cells.
