Fluorescent labeling is a biophysical term published in 2018. Fluorescent labeling is a method of selectively covalently binding or physically adsorbing a fluorescent dye (fluorescent group or fluorescein) to a certain group of the molecule (protein, peptide, etc.) to be studied, and uses the fluorescent properties of the fluorescent dye to provide information about the object being studied. The compound on which fluorescent labeling relies is called a fluorescent substance, which refers to a compound with a chemical structure of a conjugated double bond system. When irradiated by ultraviolet light or blue-violet light, it can be excited into an excited state, and when it returns to the ground state from the excited state, it emits fluorescence. The advantages such as no radioactive pollution and easy operation make the application of fluorescent labeling in many research fields increasingly widespread.
