The MTT assay is widely used in cell biology for the study of growth factors, cytokines, nutrients, and for the screening of cytotoxic or chemotherapeutic agents. Since the number of assay steps has been minimized as much as possible, MTT assay is easy to set up and convenient. Another great advantage is that this assay yields a relatively low background. Thus, MTT assay is of high sensitivity and accuracy for the quantification of cell proliferation.